cysticfibrosis transmembrane conductance regulator
OMIM *602421
Cytogenetic location: 7q31.2
6132bp mRNA
195703bp gene
1480 amino acids
ATPase
Randak et al. (1997) expressed NBF2 of CFTR as a soluble protein fused to maltose-binding protein in E. coli and found that it catalyzed hydrolysis of ATP to form ADP and Pi. The ADP product inhibited ATPase activity. NBF2 also hydrolyzed GTP to GDP and Pi. In the presence of AMP, however, the ATPase reaction was superseded by adenylate kinase activity, resulting in formation of 2 ADP molecules from ATP and AMP. Randak et al. (1997) identified a typical adenylate kinase-like AMP-binding site in NBF2.
